ATCC’s reference-grade microbial genomes have even greater analytical power when considered in context of other closely related genomes in our database. To measure relatedness between our published genomes, we implement the most widely used approach: average nucleotide identity (ANI). In this framework, ANI values greater than 95% between two genomes indicates that these genomes are derived from members of the same prokaryotic species .
Goris J, et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. International Journal of Systematic and Evolutionary Microbiology, 57(1): 81–91, 2007. PubMed: 17220447