Welcome! You have recently purchased a Twist Comprehensive Virus Research Panel from Twist Bioscience. Now we're going to walk you through using One Codex to analyze next-generation sequencing (NGS) data generated from that panel. Collectively the Twist Comprehensive Virus Research Panel and One Codex solution will provide you with straightforward analysis of respiratory viruses for your samples of interest.
Register for an Account (if needed)
One Codex is a platform that lets you analyze next generation sequencing data from microbial samples. If you don't have an account, you can sign up for free now.
Just enter some basic contact info, pick a password, and start analyzing data!
Uploading Sample Data
You can add sequencing data generated from the Twist Comprehensive Virus Research Panel workflow into your One Codex account by navigating to the Upload page, and simply dragging and dropping your FASTQ file(s) directly into the One Codex website.
If you get stuck uploading your data into our platform, send us a note using the chat on this page or in the app and we'd be happy to help.
Analyzing your Twist Comprehensive Virus Research Panel data
After you upload your data, you can run the Twist Comprehensive Virus analysis pipeline by visiting the Run Analysis page. Here you can select one or more samples by using the search bar on the left.
Once you have a set of samples selected, click "Run" on the Twist Comprehensive Virus Research Panel analysis.
Depending on the size of your FASTQ file, you may need to wait a few minutes for these to finish processing.
Accessing your results
You can view the results of your Twist Comprehensive Virus Research Panel by clicking "View Results" from the Samples page. This brings you to a PDF report that shows genome coverage plots for all of the viruses from the panel that have been detected (with over 20% of the genome observed with greater than 10x sequencing depth) or have indeterminate results (with over 5% of the genome covered).
An example of the PDF report generated from the Twist Comprehensive Virus Research Panel.
You can take a closer look at an example report here.
If you have any questions, reach out to the One Codex team through the chat box at the bottom-right of your screen.
You can read more about the specific analysis workflow for this report here.